The Multiplatform Utility of Human iPSC-Derived Neuronal Models to Provide Complex Biological In Vitro Systems for Drug Discovery

The poster The Multiplatform Utility of Human iPSC-Derived Neuronal Models to Provide Complex Biological In Vitro Systems for Drug Discovery presents Axol Bioscience’s approach to building advanced neuronal models using human iPSC-derived cell types across multiple microphysiological platforms.

Key highlights include:

• Platforms Used:
  • 2D MEA – NETRI Neurofluidics™ DuaLink with Axion Maestro Pro
  • 3D MEA – Axion Maestro Pro with bioprinted scaffold
  • 2D Microfluidics – Xona Microfluidics® system
• Functional Assays:
  • MEA recordings of motor and sensory neurons
  • NMJ co-culture models with skeletal muscle
  • ALS motor neurons (C9orf72) show hyperexcitability and increased contraction frequency
• Matrix Optimization:
  • Sensory neurons: PDL + SureBond XF, PLO + SureBond XF
  • Motor neurons: Vitronectin + SureBond XF, PLO + SureBond XF
• Markers Validated:
  • Sensory neurons: β3 Tubulin, Nav1.7, Nav1.8, VR1
  • Motor neurons: β3 Tubulin, HB9, ChAT, ISL-1
  • NMJ: NeuN (motor neurons), α-Bungarotoxin (synapse), Titin (muscle)
• Surface Chemistry & Compatibility:
  • PDMS treatments (UV, PVA) improve fluid dynamics and cell distribution
  • Scaffold-based 3D NMJ model enables motor neuron-driven muscle contraction

This multiplatform strategy supports scalable, human-relevant models for neurodegeneration, pain, and ALS research.

Featured Axol Products:

• axoCells™ Motor Neurons – ALS (C9orf72) – ax0074
• axoCells™ Motor Neurons – Healthy Donor – ax0076
• axoCells™ Sensory Neuron Kits – ax0055, ax0555, ax0157
• axoCells™ Co-Culture Media & Supplements –