Characterization of axoCells™ iPSC-Derived Cortical Inhibitory Interneurons

The poster Characterization of axoCells™ iPSC-Derived Cortical Inhibitory Interneurons presents Axol Bioscience’s development and validation of human iPSC-derived interneurons for functional neuroscience applications.

Key highlights include:

• Differentiation Protocol:
  • Small molecule monolayer technique
  • 20-day maturation from cryopreserved progenitors
• Marker Validation:
  • ICC and RNAseq confirm expression of NKX2.1, Parvalbumin, Somatostatin, Tyrosine Hydroxylase, GAD2, and GABA
• Electrophysiological Profiling:
  • MEA recordings show spontaneous firing and inhibitory modulation in co-culture
  • Co-culture with cortical neurons (axo0015) shows reduced burst frequency and increased duration
• Functional Assays:
  • IncuCyte® Neuroburst Orange used to visualize calcium signaling
  • Co-culture demonstrates more synchronous activity than monocultures
• Subtype Composition:
  • Mixed population of interneurons including major subtypes (PV+, SST+)
  • GABAergic nature confirmed by neurotransmitter and enzyme markers

This model supports drug screening, disease modeling, and network activity studies in human-relevant systems.

Featured Axol Products:

• axoCells™ Cortical Inhibitory Interneurons – ax0667, ax0662
• axoCells™ Neural Stem Cells – ax0015
• axoCells™ Enriched Cerebral Cortical Neuron Media Kit – ax0175